Zen support software pdf

Privacy Statement. See System Requirements. Available on HoloLens. People also like. FM Radio Free. Office Free. QR Scanner Plus Free. Features Markup with highlight, underline, strikeout tools. Merge multiple files into one PDF. Split a large PDF into small pieces. Secure PDFs with passwords protection.

Remove password protection from PDFs. Fill out forms right in the PDF files. Insert stamps, images, hyperlinks to PDFs. With the Time Lapse module, damage is no longer caused to specimens as a result of unnecessarily long exposure times — an important requirement for your quantitative analyses of living specimens. Your most impressive images can be exported at any time into common video formats, such as AVI or MOV, for demonstrations and publications.

The advantage: images are acquired with maximum speed and are only limited in size by the free space available on your hard drive. With the Manual Extended Focus module you use ZEN to create images without being restricted by your microscope's depth of field. The software module extracts the sharp details at different focus positions from the interactively acquired Z-stacks and combines them into an image with maximum depth of field.

Using the live image display, additional images can be added to the intermediate result. The advantage: you always have maximum depth of field in all regions of your images. This even applies irrespective of the contrast technique used — whether transmitted light, reflected light or fluorescence, thanks to our wavelet algorithm.

With the Autofocus module for widefield systems you determine the optimal focus position for your specimen in transmitted light, reflected light and reflected light fluorescence.

ZEN "learns" the ideal position during your everyday work on the light microscope and is therefore trained continuously with regard to correct focusing.

No calibration procedure is required for this and the focus is recorded automatically for all objectives and contrast techniques. The additional advantage for you: in the case of images that are acquired over time or at different positions, the system continually refocuses automatically. This ZEN module is ideal if your specimens no longer fit into the image field. With Panorama you create high-resolution overview images from individual images.

The software puts the images together with pixel precision. The advantage: even images that have been acquired offset from each other can be joined precisely using ZEN and Panorama. This means that important details from the individual images of your microscope specimen are recorded in a single image. A special feature here is that you can use the live image as a 'joystick' and therefore acquire the individual images even more quickly.

Processing Images With these powerful additional modules you can display fluorescence signals with even greater contrast or visualize image stacks in 3 dimensions. ZEN therefore creates the best possible conditions for your experiments and publications. The Colocalization module can be used to unmix the overlapping signals of different fluorescence channels.

To do this, the ZEN software makes use of modern methods, such as the automated threshold value method of Costes et al. Meaningful results on the colocalization of proteins can be obtained as a scatter plot , image and data table.

If you only want to analyze certain areas of the image, you can simply mark the corresponding region directly in the image and scatter plot.

Spectral Colocalization for ZEN then supplies the data both for the whole image and the selected regions. Of course you can also export your measured values in CSV format and process them further using a spreadsheet.

Due to the optical properties of the microscope system, light from the areas above and below the focal plane also enters the image. This means that fine structures on the specimen can no longer be picked out. It is precisely here that the 3D Deconvolution software module starts, by calculating out of focus light back to its place of origin.

The distorted specimen structure unfolds and is displayed more sharply. This technique also reduces any image noise that may be present, making a decisive contribution to the first-class image quality of your image stacks and 3D models.

With 3Dxl you can display multi-dimensional image stacks in 3D. Highlight is its ability to handle really large images hundreds of gigabyte efficiently and smoothly. You can position a three-dimensional model interactively according to the view that you require and provide it with annotations, coordinates and scaled axes.

It goes without saying that 3Dxl also provides you with tools for the interactive measurement of distances and angles. Would you like to present or publish the result afterwards?

Analyzing and Interpreting Image Data ZEN provides important information on your microscope specimens interactively or fully automatically. Besides size and volume information, this also includes detailed data on cell physiological processes. Would you like to generate automatic measurement procedures simply yourself? The ZEN Image Analysis module makes it possible for you to do this, even without programming knowledge. With our measurement program wizard you tackle even complex measurement tasks yourself in just a few minutes.

Once created, the programs are always available to be used for an unlimited number of images. The one should be on the bottom right, appeared on the top right. Is it due to the stage settings or the way this application merge the images?

Hi there — thank you for contact us! Your local product team is the best group to contact for your software question. Here is their contact information:. Thank you for your question. Here is the contact information:. We apologize that we cannot assist you through this platform. Service and support questions such as these are coordinated by the local team. We encourage you to reach out to them. Here is the contact information for your local office:.

I have spent hours trying to find an updated guide for the Image Analysis Module and the best I am getting is something from , which is pre 2.

Dear Visitor unfortunately this blog post is no longer monitored. The histogram provided by the ZEN 2. In the case of intensity, what do the values mean?